Production and Optimization of Thermostable lipase from a Thermophilic Bacillus sp LBN 4

Abstract

The Bacillus sp isolated from the hot spring of Tawang district of Arunachal Pradesh, India was studied. Maximum enzyme production was achieved after 20 Hrs of cultivation. The olive oil and yeast extract was found to be the most suitable substrate for enzyme production. The enzyme had temperature optima of 65°C. Over 80% of its peak activity was at the pH range of 7-8, with an optimum of 8.0. The enzyme was found to be most stable at 80°C for 10 minutes. Almost 80% of the residual activity was retained at 80°C. CaCl2 was found to stimulate the lipase activity by 40%.