In Vitro Propagation of Saintpaulia ionantha Wendl 1

Abstract

Abstract Small leaf-sections of African violet proliferated rudimentary shoots when explanted on a modified Murashige and Skoog's (MS) high salt medium, containing 5.4 μ M naphthalene-acetic acid (NAA), 22 μ M 6-benzylamino purine (BA), and 308 μ M adenine sulfate. The subdivided rudimentary shoots grew and rooted after subculturing onto a modified Morel and Muller (MM) mineral salt medium, lacking growth regulators and having a reduced sucrose concentration. Rooted plants reached the flowering stage after 4 months of greenhouse culture. A single leaf can produce approximately 500 plants, having the desirable habit of a flat-leafed rosette and the capacity to flower within about 7 months.