Regeneration of Acacia melanoxylon plantlets in vitro

Abstract

Callus from juvenile material of mature Acacia melanoxylon R. Br. trees formed shoots on a Murashige and Skoog’s medium when supplemented with benzyladenine (1 μM) and indole-3-acetic acid (1 μM). lndole-3-butyric acid at 10 μM was the best treatment for the induction and growth of roots on the in vitro-produced shoots. Kallus wat gevorm is deur jeugdige materiaal van Acacia melanoxylon R. Br. het lote gevorm op ’n medium van Murashige en Skoog in die teenwoordigheid van bensieladenien (1 μM) en indool-3-asynsuur (1 μM). Die optimale behandeling vir die induksie van wortels op die in vitro-gekweekte lote was indool-3-bottersuur teen ’n konsentrasie van 10 μM.