PubMed · 1996 · 24 citations · 0 references
HumanitiesArchaeologyMolecular BiologyParasitologyProtozoologyZooarchaeologyAncient DnaBiomolecular ArchaeologyChagas DiseaseMolecular Diagnosis Of ParasitesAncient Dna IdentificationDetection Of Protozoan ParasitesDna Extraction From Degraded SamplesSpecies Identification From Ancient DnaTrypanosoma Cruzi InfectionTrypanosome Detection
The polymerase chain reaction (PCR) has been used for clinical diagnosis of infectious disease and to research ancient animal and microbiological DNA from a wide range of tissues. PCR was used to study the possibility of Trypanosoma cruzi kinetoplast DNA (kDNA) extraction from experimentally desiccated mouse tissue (heart, skeletal muscle, liver, spleen, and pancreas). The results obtained suggest the application of this technique to T. cruzi detection in archaeological material.