Preparation of Rolled Epidermis of Vicia Faba L. So That Stomata are the Only Viable Cells: Analysis of Guard Cell Potassium by Flame Photometry

Abstract

A rolling technique is described with which cells in epidermal strips of V. faba were differentially broken so that only guard cells remained alive and functional. In rolled epidermis potassium was retained only in live guard cells, as judged by staining with cobaltinitrite.