The retention behavior of reversed-phase HPLC columns with 100% aqueous mobile phase

Abstract

The authors studied the retention behavior of reversed-phase high performance liquid chromatography (HPLC) columns under 100% aqueous mobile-phase conditions. They report about the behaviors of reversed-phase HPLC columns such as C18 and C8 columns and discuss why they exhibit decreased retention times. They also suggest criteria for achieving reproducible results from reversed-phased columns under highly aqueous mobile-phase conditions.