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Histamine release induced by pituitary adenylate cyclase activating polypeptide from rat peritoneal mast cells.
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1994
Year
Mast Cell DisorderPacap 38Experimental PharmacologyCellular PhysiologyGastrointestinal Peptide HormoneMolecular PharmacologyPituitary GlandPacap 27Cell SignalingPituitary Adenylate CyclaseMolecular PhysiologyAllergyEndocrine MechanismEndocrinologyPharmacologyHistamine ReleaseSignal TransductionPhysiologyMedicineIncreased Histamine ReleaseNeuropeptides
Pituitary adenylate cyclase activating polypeptide 38 (PACAP 38) and its fragments PACAP 27, PACAP 16-38, PACAP 28-38, PACAP 31-38 were compared for their histamine releasing effects on rat peritoneal mast cells. PACAP 38 and PACAP 16-38 were the most active peptides, followed by PACAP 27. PACAP 38 and PACAP 16-38 dose-dependently increased histamine release at a concentration of 1 x 10(-8) mol/l or higher, and these releasing activities were more than 100 times more potent than that of substance P. Extracellular calcium inhibited the substance P-induced histamine release. In contrast, PACAP 38- and PACAP 27-induced histamine releases were hardly inhibited by extracellular calcium.