METHOD FOR THE ASSAY OF ANTIDIURETIC HORMONE IN PLASMA WITH A NOTE ON THE ANTIDIURETIC TITER OF HUMAN PLASMA.

Abstract

Abstract A method for the extraction and purification of antidiuretic hormone (ADH) from human plasma is described. Share's original procedure involves the trichloroacetic acid extraction of ADH from plasma, ether wash, and purification of the extract with XE-64 resin column chromatography followed by the ADH assay in water-loaded, ethanol-anesthetized rats with changes in conductivity of the urine as the index of response. This was modified by the use of 50 per cent acetic acid instead of pyridine acetate for the elution of ADH from a XE-64 resin column yielding a better recovery of ADH from plasma. ADH levels in normal human plasma averaged 1.9 μU per milliliter by this method; they were 6.5 μU per milliliter after dehydration.