A Solid Liquid State Culture Method to Stimulate Monascus Pigments by Intervention of Different Substrates

Abstract

The present study illustrates the investigation carried out on the production of pigment by Monascus sanguineus and Monascus purpureus MTCC 410 under solid state and submerged conditions. Also, in addition to that, various aspects of pigments, the effect of physical and nutritional factors and different substrates for enhanced pigment production were studied. Growth pattern of M. purpureus and M. sanguineus was studied in tryptone glucose yeast extract and it was found that maximum biomass and pigment yield was noticed on 15 th day of incubation in this growth medium and dry weight obtained was 2.25g/l. Screening of substrates for both the sp. was also carried out. Both sp. were able to grow in all the experimented substrates. Coconut residue was found to be the best substrate for Monascus purpureus MTCC 410 whereas Potato peel was recognized to be the best substrate for Monascus sanguineus. Different polar and non polar solvent were used for extraction of pigment and among them methanol was found to be best solvent for extraction of red pigment.