European Journal of Gastroenterology & Hepatology · 1993 · 14 citations · 0 references
Veterinary MedicineImmunologyImmunotoxicologyInflammationMucosal ImmunologyGastrointestinal InflammationMechanisms Of Toxic InjuryMucosal InflammationOxidative Damage To BiomoleculesOxidative Stress In InflammationPeptic Ulcer DiseaseGastric InjuryGastrointestinal Mucosal DamageHelicobacter Pylori InfectionHelicobacter Pylori PathogenesisMucosal CytotoxicityMucosal Oxidative StressMucosal Tissue DamageNeutrophil-mediated Mucosal Damage
Purpose: To investigate Helicobacter pylori-dependent neutrophil activation and the in vitro effects of neutrophil-derived toxic oxidants on gastric mucosal cell injury. Methods and results: The luminol-dependent chemiluminescence activity of human neutrophils increased significantly after H. pylori was added, indicating that the oxidative burst of neutrophils was actually conducted by H. pylori. The cytotoxicity of cultured gastric mucosal cells was assessed by the release of 2,7-bis-carboxyethyl-5(6)-carboxyfluorescein fluorescence from cellular cytoplasm. Neither H. pylori nor neutrophils alone produced significant epithelial cell injury but considerable damage was inflicted by H. pylori-activated neutrophils