Urea Biosensor Based on the Composite Film of Electroinactive Polypyrrole and Urease Modified with Polyanion

Abstract

A urea sensor based on the composite mm of electroinactive polypyrrole(PPy)and ureasc(Urs)modified with polyanion wsbricated by electropolymcrizationThis sensor showed the potentiahesponse to urea based on the detection of pH-change during the enzymatic reactiQn by UrsThe scnsltivity of this scnsof was53mVdecade l which vs higher than that ofthe PPy film coated electrode in which Urs was entrapped physicallyaspreviouslyreportedThehighsensitlvitywasconsideredtobeattributedtotheincreasein the number of immobilized Ufs with the covalent bonding with carboxyl groups ofPAAThis urea sensor showed hncar potential fcsponsc between l10 4mol dm 3and l X10-L mol dm-3