Cloning of a Gene Suppressing Hydrogen Sulfide Production by <i>Saccharomyces Cerevisiae</i> and its Expression in a Brewing Yeast

Abstract

NHS5, a gene suppressing formation of hydrogen sulfide (H2S), was cloned from a Saccharomyces cerevisiae X2180-1A gene library. The gene was inserted into suitable vectors and introduced into bottom-fermentation yeasts. We verified that the strain with the inserted gene was effective in beer production because the gene reduced H2S content in green beer and did not affect other fermentation characters. We also proved that the function of the gene was related to production of cystathionine β-synthase.