Determination of motility forces of human spermatozoa using an 800 nm optical trap.

Abstract

The measurement and calculation of trapping forces on ellipsoidal specimens, and the determination of intrinsic motility forces of human spermatozoa by employing an 800 nm optical trap ("laser tweezers"), are presented. ATP-driven motility forces were calculated from calibrated trapping forces generated during the interaction of an 800 nm laser beam with single sperm cells. Calibration was performed by moving optically trapped sperm heads through a laminar fluid and calculating viscous forces for an ellipsoidal cell shape. Sperm heads were obtained by microsurgically removing flagellum with a pulsed laser beam ("laser scissors"). A trapping efficiency of 0.12 +/- 0.02 and a mean intrinsic motility force of 44 +/- 20 pN were determined for motile spermatozoa from healthy donors.