Ninhydrin-Schiff and alloxan-Schiff staining; a new histochemical staining method for protein.

Abstract

Abstract A new histochemical test for protein and peptide, based on the liberation aldehyde groups by ninhydrin or alloxan oxidation, and subsequent demonstration of aldehyde by reduced fuchsin, has been described. Experiments of enzymatic digestion and spot test showed the specificity of this reaction. It was compared with three present histochemical techniques for protein, and proved to be preferable in many respects. This method, named A.S. or N.S. stain, gives a distinct color reaction, according to the distribution of protein in tissues.