Alterations of Ca++ uptake and lipid content in cultured human arterial smooth muscle cells treated with 26-hydroxycholesterol.

Abstract

The effects of 26-hydroxycholesterol on 45Ca++ uptake, [14C]cholesterol uptake, [14C] acetate incorporation and the concentrations of 26-hydroxycholesterol and cholesterol in the plasma membrane were studied in cultured human smooth muscle cells isolated from umbilical arteries. The results showed that: (1) 26-hydroxycholesterol enhanced markedly 45Ca++ uptake and the enhancement was not diminished by nifedipine; (2) 26-hydroxycholesterol decreased cholesterol content in plasma membrane by inhibiting cholesterol uptake and synthesis, and [14C]cholesterol uptake was not LDL-receptor dependent; (3) 26-hydroxycholesterol induced a shift of [14C] acetate from cholesterol into phospholipid synthesis, but the radioactive incorporation into triglyceride and cholesterol ester was inhibited by 26-hydroxycholesterol; and (4) 26-hydroxycholesterol inserted itself into the plasma membrane. We suggested that 26-hydroxycholesterol changes the composition of membrane lipids with a consequential alteration of membrane permeability to Ca++.