2011 · 29 citations · 8 references
PharmacyPharmaceutical AnalysisPharmaceuticsBiopharmaceuticsChromatographic AnalysisMass Spectrometric AnalysisAnalytical ChromatographyBioanalytical Method ValidationChromatographic Analysis Of PharmaceuticalsChromatographyLiquid ChromatographyChromatographic Drug AnalysisPotassium Dihydrogen PhosphateRecovery StudiesTablet Chromatographic Analysis
A simple, accurate, sensitive and validated RP‐HPLC method for simultaneous determination of Cefixime and Ofloxacin in combined tablet dosage form has been developed. Separation was carried out on Jasco HPLC system equipped with HiQ‐SiL C8 Neosphere column (150 × 4.6 mm i.d.) and UV/VIS detector using Methanol: 0.025 mM potassium dihydrogen phosphate buffer in ratio of (70:30, v/v) as mobile phase and detection was carried out at 290 nm. Ambient temperature conditions were maintained. Results were linear in the range of 1‐10 µg/mL for both the drugs. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.
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