Enzymes of Aspergillus Oryzae III. The Sequence of Appearance and Some Properties of the Enzymes Liberated During Growth

Abstract

When a strain of the mould Aspergillus oryzae is grown in surface culture on a protein-free sucrose-tartrate medium, enzymes are released into the culture medium in the following order: first, the carbohydrases sucrase and amylase together with phosphatase, second, the proteases and esterases, and third, catalase. Their liberation is accompanied by the appearance of protein nitrogen in the medium and loss in weight of the mycelium.