Relative sensitivity of DNA and photosystem II in Ulva intestinalis (Chlorophyta) under natural solar irradiation

Abstract

High intensities of sunlight can result in DNA and photosystem II (PSII) damage. However, the relative sensitivity of both these targets under natural sunlight and especially over a long period has not been studied in algae so far. Although DNA damage is highly specifically induced by ultraviolet-B radiation (UVB, 280-315 nm), PSII is inactivated by a broad spectrum. The green macroalga Ulva intestinalis is an appropriate and interesting study organism with which to investigate the relative importance of the 2 different targets of sunlight because this alga contains no UV-screening protective pigments, although it is exposed to strong solar irradiation in its natural habitat. This entails a high risk of DNA damage. Therefore, diel time courses and long-term development of DNA damage and the optimal quantum yield of PSII (F-v/F-m) were studied in situ. F-v/F-m was extremely reduced at noon, but a fast recovery was observed in the afternoon. As dark-adapted basal fluorescence (F-o) of PSII was substantially decreased during the day, non-photochemical quenching is suggested to be a key photoprotective strategy in U. intestinalis. In contrast, even in samples with strongly reduced F-v/F-m, only very low DNA damage was found, irrespective of the accumulated UVB dose. We propose that efficient photoreactivation driven by natural sunlight balances the induction of dimers. This leads to a higher UVB tolerance of DNA than that observed in algae under experimental UVB irradition. In this field study, U. intestinalis suffered more from photoinhibition than from DNA damage.