Capillary electrophoresis enantioseparation of noncharged and anionic chiral compounds using anionic cyclodextrin derivatives as chiral selectors.

Abstract

The electrophoretic profiles of anionic cyclodextrin derivatives were studied in benzoic acid and phosphate buffers. All anionic derivatives studied exhibited a multicomponent profile. The mobilities of beta-cyclodextrin (beta-CD) sulfates do not depend substantially on the pH. Carboxymethyl ether of beta-cyclodextrin (CM-beta-CD) possesses marked self-mobility at pH 4.5, and as a result, it separates a number of neutral analytes at this pH. Sulfated beta-CD derivatives also exhibit chiral recognition abilities toward neutral compounds, and additionally, the anionic sulfobutyl ether of beta-cyclodextrin (SBE-beta-CD) successfully resolves the chiral solutes warfarin and acenocoumarol also in the anionic form.