An HLA-linked gene controls susceptibility to lepromatous leprosy through T cell regulation

Abstract

Genetic control of the clinical manifestation of leprosy was investigated in 66 unrelated pa tients with leprosy and 8 multiplex families. In 32 lepromatous leprosy(LL) patients, both phenotype frequency of HLA-DR2 and haplotype frequency of HLA-B35-DR2-DQwl were significantly increased. Our family data combined with other investigators' showed that the distribution of shared HLA haplotypes differed significantly from the random distribution, thereby suggesting the existence of an HLA-linked major gene for lepromatous leprosy. To investigate the function of this major gene, the cellular mechanism of nonresponsiveness of LL that is strictly specific to mycobacterium leprae (ML) antigen was analysed using pan ning technique and monoclonal antibodies. We have tested 30 LL patients for their suppres sive activity of T8 cells on the T cell response to ML of tuberculoid leprosy(TT) patients. T8 cells from two LL patients abrogated the response of TT patients. None of LL patients tested showed proliferative response to ML antigen even when we removed the T8 cells from the culture . Therefore, we concluded that nonresponsiveness to ML antigen of LL patients in vit ro were generated by the elimination of responding T cells. In the minor population of LL, T8 suppressor T cells were still active in peripheral blood. The T8 suppressor T cells might play some role in the elimination of responding T cells to ML antigen.