Comparative flavoprotein catalysis of anthracycline antibiotic. Reductive cleavage and oxygen consumption.

Abstract

Several flavoenzymes are compared for their ability to catalyze reductive glycosidic cleavage of anthracycline antibiotics and to promote oxygen consumption in the presence of the antibiotics. NADPH cytochrome P-450 reductase, xanthine oxidase, nitrate reductase, NADH cytochrome c reductase, and lipoamide dehydrogenase catalyzed with decreasing specific activity the reductive glycosidic cleavage of daunorubicin and showed enhanced oxygen consumption in the presence of daunorubicin. Gluthione reductases, L-amino acid oxidase, D-amino acid oxidase, D-glucose oxidase, and lactic dehydrogenase were inactive. All of those flavoenzymes which catalyze reductive glycosidic cleavage possess single-electron transfer systems.