Development of respiration in yeast grown anaerobically on different carbon sources

Abstract

REACTIONS OF N-ETHYLMALEIMIDE 595 the folding of a peptide chain within a protein may decrease the reactivity of a-or e-amino groups by rendering them less accessible; on the other hand, charged groups may be close to a particular amino or imidazole group, and thereby enhance its reactivity. The distinction between 'reactive' and 'unreactive' groups in a protein is best established by a careful investigation, preferably involving degradation of the modified protein and analytical studies on the isolated peptides. In this way, the extent and sites of a reaction can be determined with precision. Methods involving measurement of the disappearance of the reagent SUMMEARY 1. The rates of reaction of N-ethylmaleimide with the a-amino groups of three peptides are reported.