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Comparison of Whole Blood and Purified Canine Lymphocytes in a Lymphocyte-Stimulation Microassay
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1978
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ImmunologyVeterinary ResearchEducationWhole BloodLaboratory Animal StudyHematologyDifferent MitogensLymphatic SystemPurified Canine LymphocytesLaboratory MedicineAnimal PhysiologyVeterinary PhysiologyAllergyVeterinary PathologySmall Animal Internal MedicineLaboratory Animal CareLymphocyte-stimulation MicroassayAnimal SciencePhysiologyVeterinary ScienceMedicineOptimum Mitogen Concentration
SUMMARY The optimum mitogen concentration and time required for using whole blood from dogs in a microassay were determined, and this test then was compared with a standard lymphocyte-stimulation microtest, using gradient-isolated lymphocytes, 2 different mitogens (phytohemagglutinin and concanavalin A), and 2 different culture media. Statistical analysis of the data from 10 dogs showed that whole blood was significantly more reactive than were gradient-isolated lymphocytes ( P < 0.05). Waymouth's medium was significantly better than RPMI 1640 ( P < 0.001), and concanavalin A was significantly more mitogenic than phytohemagglutinin ( P < 0.001). The interaction between lymphocyte source and mitogens was the only one of the various interactions that was significant at P < 0.05.