Ubiquinone content and respiratory burst activity of latex-filled phagolysosomes isolated from human neutrophils and evidence for the probable involvement of a third granule.

Abstract

Human neutrophils prepared from fresh blood were activated with either latex o r latex plus phorbol myristate acetate.Phagolysosomes subsequently isolated by gradient centrifugation were enriched 6-o r 4-fold, respectively, in respiratory burst activity as measured by superoxide dismutase-sensitive NADPH-cyto-