LM-Ag and LSP--two different target antigens involved in the immunopathogenesis of chronic active hepatitis?

Abstract

Two different types of immune reactions against liver membrane antigens have been described: firstly, cell-mediated immunity and autoantibodies against the liver-specific protein (LSP) in HBsAg-negative and HBsAg-positive chronic active hepatitis (CAH), and secondly, a liver membrane autoantibody (LMA) in HBsAg-negative CAH. Using 100,000 g supernatants of human and rabbit liver homogenates, the corresponding antigen of LMA could be separated by affinity chromatography on insolubilized LMA-positive sera from patients with HBsAg-negative CAH. A further characterization by crossed immunoelectrophoresis showed that LMA is directed against a soluble liver membrane antigen (LM-Ag) that is not a constituent of the purified LSP. LM-Ag seems to be species-unspecific and moves faster than LSP and serum albumin in crossed immunoelectrophoresis. While LSP is believed to be involved in the pathogenesis of HBsAg-negative and HBsAg-positive chronic active hepatitis, LM-Ag may be a target antigen only in cases of HBsAg-negative autoimmune CAH.