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Amiloride-sensitive net Mg2+ efflux from isolated perfused rat hearts.
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1987
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Molecular PharmacologyCardiac MuscleCardiovascular DiseaseMedicinePhysiologyCardiovascular PharmacologyIon ChannelsNet Mg2+ EffluxVascular BiologyPharmacotherapyElectrophysiologyCardiovascular PhysiologyPerfused Rat HeartsMg2+ ConcentrationPharmacologyMg2+-free SolutionCardiology
Coronary blood vessels of isolated spontaneously beating rat hearts were perfused with an Mg2+-free solution at a rate of 3 ml/min. 1.5-ml fractions were sampled. Net Mg2+ efflux from heart muscle cells was measured by the increase of Mg2+ concentration in the perfusate. Addition of 0.3 microM isoproterenol to the perfusate resulted in a rapidly occurring transient Mg2+ efflux which was inhibited by amiloride.