Publication | Closed Access
Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport
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Citations
22
References
2015
Year
Molecular BiologyAnalytical UltracentrifugationChemical BiologyReversible ControlProtein X-ray CrystallographyMolecular RecognitionComputational BiochemistryProtein ChemistryBiochemistryG Protein-coupled ReceptorMedicineBiomolecular InteractionProtein TransportNon-peptide LigandProtein LocalizationMolecular ModelingFast Dissociation KineticsStructural BiologyBiomolecular EngineeringArtificial LigandsNatural SciencesIntracellular TraffickingMolecular DockingIntracellular Protein TransportSmall MoleculesDrug Discovery
Artificial ligands of streptavidin (ALiS) with association constants of ∼10(6) M(-1) were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.
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