Human X human hybridoma producing monoclonal antibody against autologous cervical carcinoma.

Abstract

A stable human X human hybridoma, termed CLNH11, was produced by fusing UC 729-6, a 6-thioguanine-resistant human lymphoblastoid B-cell line, with lymphocytes obtained from a patient with squamous cell carcinoma of the cervix. CLNH11 could grow both in serum-containing and serum-free media, and produced a significantly higher titer of antibody in serum-free media. CLNH11 secreted a human monoclonal immunoglobulin G which bound to the autologous cervical carcinoma in both tissue sections and primary cultured cells, the cervical cancer cell lines Hela and CaSki, but not to normal fibroblasts.