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Use of a novel agarose gel-digesting enzyme for easy and rapid purification of PCR-amplified DNA for sequencing.
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1992
Year
Rapid PurificationDna AnalysisMolecular BiologyNucleic Acid Amplification TestPcr ProductsPoint Agarose GelsGenomicsPcr-amplified DnaBioanalysisChromatographyDna SequencingMolecular Biological MethodDna ReplicationSequencingNatural SciencesBiotechnologyGenetic EngineeringNucleic Acid AmplificationMicrobiologyMedicine
The use of a novel gel-digesting enzyme preparation provides an easy, rapid and convenient method to quantitatively recover PCR-amplified DNA from low melting point agarose gels. The PCR products purified using this method were readily sequenced and yielded good and unambiguous sequence data.