Massenspektrometrische Identifizierung der Phenylthiohydantoin-Derivate von Aminosäuren / Identification of Amino Acid Phenylthiohydantoins by Mass Spectrometry

Abstract

The unequivocal identification of phenylthiohydantoins of the 20 amino acids usually occurring in proteins is possible by mass spectrometry and by combined gas chromatography-mass spectrometry. The method is sensitive enough to be applied to the identification of phenylthiohydantoins obtained from automated Edman degradations of polypeptides. Mass spectra of 21 amino acid phenylthiohydantoins are presented and interpreted.