Publication | Closed Access
Preparation of recombinant coat protein of Prunus necrotic ringspot virus.
11
Citations
0
References
2001
Year
Virus StructureMolecular VirologyRecombinant Coat ProteinCoat ProteinPathogenesisImmunologyEscherichia ColiVirologyMicrobiologyPet 16BViral Structural ProteinMedicineViral Genetics
The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).