Pyridoxamine phosphate-oxidase and pyridoxal phosphate-phosphatase activities in <i>Escherichia coli</i>

Abstract

first demonstrated the conversion of pyridoxamine phosphate into the aldehyde form by cell-free extracts of Escherichia coli. The nature of the product was establiBhed by its ability to activate apotryptophanase, an enzyme also present in the aqueous extracts. Optimum conditions for enzyme assay were studied, and simple kinetic studies were carried out by spectrophotometric methods, by Beechey & Happold (1957), who found that E. coli extracts