PubMed · 1992 · 43 citations · 0 references
Life SciencesBiochemistryImmunologyClinical ImmunologyProtein BiochemistryProtein-protein InteractionsAntibody CharacterizationAntiphospholipid SyndromeAutoantibodiesAnti-beta-2 Glycoprotein I AntibodiesAnti-phospholipid AntibodiesAntibodiesAnticardiolipin AntibodiesAntiphospholipid Syndrome TestingCardiolipin-protein InteractionsImmunoassays For Apolipoproteins
IgG was isolated from 9 patients with high levels of anticardiolipin antibodies (aCL). The standard ELISA was modified, using gelatin to postcoat ELISA plates and as diluent. Under these conditions, 8/9 samples of IgG bound to cardiolipin only in the presence of a cofactor, found in fetal calf serum and normal human serum (NHS). beta 2-glycoprotein I (apolipoprotein H) was at least as effective as NHS as a cofactor for 7/9 IgG samples. Our studies confirm that for some aCL detected in conventional assays, the antibody will react with cardiolipin only if beta 2-glycoprotein I is present.