Spectrophotometry of proteins

Abstract

MEASUREMENTS of the absorption spectra of the aromatic amino-acids have been frequently reported in the literature but the results are not in good agreement. For purposes evident in Part II it was required to know the absorption curves of tyrosine and tryptophan with accuracy. Their absorption curves were therefore redetermined. The method of Vierordt for estimating spectrophotometrically the concentration of the components of a mixture was also investigated in its application to mixtures of tyrosine and tryptophan.