Glycoprotein detection on immobilon PVDF transfer membrane using the periodic acid/Schiff reagent.

Abstract

The periodic acid/Schiff (PAS) stain is a popular method for the detection of glycoproteins in polyacrylamide gels. The use of this stain after Western transfer of glycoproteins to PVDF membrane eliminates the need for extensive fixation steps, as well as shortening the time needed for washing steps. An example of the usefulness of this technique is demonstrated in the staining of a partially purified high molecular weight human tumor-associated glycoprotein antigen from the ascitic fluid of an ovarian cancer patient