The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants.

Abstract

Plants regenerated from tobacco hairy root callus cultures were fertilized by self-pollination, and healthy, morphologically normal R1 offspring were obtained. Three of these R1 seedlings were analyzed for the presence of T-DNA and synthesis of the T-DNA-specific compound agropine. All three R1 plants analyzed contained the same full-length T-DNA as the parental regenerant, while only two showed agropine synthesis.