A functional analysis of dopaminergic innervation of the neurohypophysis

Abstract

Dopaminergic neurons arising from cell bodies in the rostral arcuate nucleus of the hypothalamus have been shown to make axoaxonic contact with neurohypophyseal neurosecretory axons. In this study, electrical stimulation of the rostral arcuate nucleus depresses multiunit electrical activity recorded from neurosecretory axons within the neurohypophysis. After a single 5-s stimulus train, neurohypophyseal electrical activity is reduced to 6% of control. The superfusion of dopamine (5 micrograms/microliters) onto the neurohypophysis also has an inhibitory effect. Superfusion directly onto the neurohypophysis of the dopamine-receptor antagonist, pimozide (1 micrograms/microliters), abolishes the inhibitory effect of arcuate nucleus stimulation. These findings suggest that the dopaminergic innervation of the neurohypophysis may have an inhibitory influence on the release of neurohypophyseal hormones.