PubMed · 1992 · 17 citations · 0 references
Life SciencesCell BiologyMolecular BiologyCell AdhesionCell PhysiologyEndocytosisAnimal Cell CultureCell-matrix AdhesionExtracellular MatrixExtracellular Matrix BiochemistryExtracellular Matrix BiologyIntercellular CommunicationCell Adhesion To LamininCell Adhesion To The Extracellular MatrixCell-surface InteractionsExtracellular Matrix ProteinsExtracellular Polymeric MatrixFluorescein Staining ImagingLaminin BindingMatrix-cell Interactions
Pneumocystis carinii cells labeled with fluorescein isothiocyanate were co-cultured with tissue culture cells. Measurements of attachment was determined by the tissue culture cell fluorescence after washing out the P. carinii organisms. The effects of the extracellular matrix proteins, laminin and fibronectin, on the binding of P. carinii onto the monolayer of cultured cells were investigated for better understanding of organism-cell interactions. The internalization of P. carinii by MRC5 cells was observed.