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On the regulation of acetylcholine release: a study utilizing Torpedo synaptosomes and synaptic vesicles.
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1980
Year
Synaptic TransmissionNeurotransmitterNeurotransmissionPresynaptic Muscarinic ReceptorsCellular NeurobiologySynaptic SignalingCellular PhysiologySocial SciencesBand Alpha ProteinMembrane TransportSynaptic VesiclesSynaptic PhysiologyNeurochemistryBiophysicsMolecular PhysiologyMolecular NeuroscienceBiochemistryTorpedo SynaptosomesMembrane BiologyNervous SystemPharmacologySynaptic PlasticitySignal TransductionCellular NeurosciencePhysiologyAcetylcholine ReleaseNeuroscienceMolecular NeurobiologyCellular BiochemistryMedicine
1. Addition of ATP to isolated highly purified Torpedo synaptic vesicles results in 45Ca2+ uptake. 2. Ca2+ dependent ACh release from Torpedo synaptosomes is accompanied by the phosphorylation of a specific protein with an apparent subunit molecular weight of about 100,000 (band alpha). 3. Activation of the presynaptic muscarinic receptors by an agonist inhibits Ca2+-dependent ACh release from Torpedo synaptosomes. This process seems to be mediated through an interference with the phosphorylation of the band alpha protein, and not by blocking the voltage-dependent presynaptic Ca2+ channel.