Phosphorylation of acyclic nucleotide analogs HPMPA and PMEA in L1210 mouse leukemia cell extracts.

Abstract

Acyclic nucleotide analogs (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and 9-(2-phosphonylmethoxyethyl)adenine (PMEA) were phosphorylated in the presence of ribonucleoside 5'-triphosphates by the crude extract from mouse leukemia cells L1210 affording the respective mono- and diphosphoryl derivatives. The donor efficiency was decreasing in the order CTP greater than UTP greater than ATP greater than GTP. The presence of an ATP regenerating system stimulated considerably the conversion of both compounds. The rate of PMEA phosphorylation was 5-times slower than that of HPMPA both with and without an ATP regenerating system.