Erythropoietin Assay with Posthypoxic-Polycythemic Mice

Abstract

SUMMARY The exposure of AKR mice to 8% oxygen and 92% nitrogen for 72 hours during a period of 2 weeks caused sufficient hypoxia to produce the desired polycythemia in the mice used for the bioassay of erythropoietin. The reduction of the time of exposure to these hypoxic conditions from 136 to 72 hours improved the reproducibility and sensitivity of the bioassay.