Defining the carbohydrate specificities of Erythrina corallodendron lectin (ECorL) as polyvalent Galbeta1-4GlcNAc (II) > monomeric II > monomeric Gal and GalNAc.

Abstract

[1] Galbeta1-4GlcNAc (II) and other Galbeta1 -related oligosaccharides are essential for binding. [2] Their polyvalent form in glycoproteins is the most important binding factor for ECorL, while II monomer and oligo-antennary II forms play only a limited role in binding. [3] Although GalNAc is more active than Gal for ECorL, its reactivity is not changed by polyvalent effects. This lectin may be used as a tool to study glycobiology in basic and medical sciences.