Differential control of free calcium and free zinc levels in isolated bovine liver nuclei

Abstract

Modulation of intracellular signal transduction by Ca2+ and possibly Zn2+ is based on an effective homoeostatic control of the corresponding free ion concentrations. We used the fluorescent indicator fura-2 to monitor concentrations of free Ca2+ and free Zn2+ in nuclei isolated from bovine liver. The nuclei showed an ATP-stimulated accumulation of intranuclear free Ca2+, which was inhibited in the presence of the Ca(2+)-pump inhibitor thapsigargin. Furthermore, uptake and intranuclear levels of free Zn2+ were measured after incubation with different extranuclear Zn2+ concentrations. There was no stimulating effect of ATP on Zn2+ uptake. Our data suggest that the levels of intranuclear free Ca2+ and free Zn2+ are controlled differentially. A distinct regulation of free ion levels in the nucleus may contribute to the specific control of nuclear events associated with gene transcription and cell differentiation.