Comparison of two tandem mass spectrometry-based methods for analyzing the proteome of healthy human lens fibers.

Abstract

The data clearly indicated that the SDS-PAGE-RPLC-MS/MS method was more suitable than the SCX-RPLC-MS/MS method for analyzing lens fiber proteome. This work greatly expanded the proteome database of human lens fibers, and the results provided a reference for future studies to detect aging-related and cataract-related changes in human lens fibers proteins.