Fluorescence Analysis of Flavoproteins

Abstract

Fluorescence is one of the most powerful tools in the study of flavoproteins. It allows the user to investigate many aspects of the nature, structural integrity and kinetic properties of the flavin. In this chapter, we will outline the basics of fluorescence spectroscopy and give details of the operating procedures required to obtain different kinds of information about the protein-bound chromophore. Leading on from this will be a number of examples of the uses of fluorescence in flavoprotein analysis. In Subheading 4., the potential benefits and drawbacks of fluorescence spectroscopy in the study of flavoproteins will be discussed.