PubMed · 2008 · 59 citations · 0 references
Life SciencesImmunologyMicrobiologyCell SignalingInflammationInnate ImmunityAnti-inflammatory PharmacologyInflammation PharmacologyInflammatory SignalingMacrophage BiologyMarine Bioactive CompoundsPhagocyte BiologyBacillus SubtilisMacrophage ImmunomodulationMacrophage Inflammatory ResponsesMacrophage Responses To BacteriaMacrophage Signaling PathwaysMacrophage-mediated InflammationNuclear Translocation Of Transcription Factors
Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin downregulated LPSinduced NO production in RAW264.7 cells and primary macrophages with IC50 values of 31.6 and 22.4 microM, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and IkBa and the nuclear translocation of NF-kappaB (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived antiinflammatory agent with anti-NF-kappaB activity.