Comparative Carcinogenic Effects of <italic>N,N′</italic>-2,7-Fluorenylenebisacetamide by Intraperitoneal and Oral Routes of Administration to Rats, With Particular Reference to Gastric Carcinoma<xref ref-type="fn" rid="fn1">2</xref>

Abstract

This is a study of the carcinogenic effects of intraperitoneal injection of N,N′-2,7-fluorenylenebisacetamide (2,7-FAA) on rats of the Buffalo strain. The results are compared with those of a previous experiment in which the same carcinogen was fed to rats. By both routes of administration 2,7-FAA induced carcinomas and precancerous, atrophic, and hyperplastic lesions of the glandular stomach. For the first time, to our knowledge, injection of a chemical carcinogen at a remote site induced gastric adenocarcinoma experimentally in rats. The carcinogenic effects of the 2,7-FAA in the two experiments were about the same for the glandular stomach, intestines, lung, and adrenal gland, and in the female, for the ear structures. By the intraperitoneal route the effect was greater for the mammary glands, skin, and liver, but was less for the salivary glands, pancreas, hematopoietic tissues, and uterus, and in the male, for the ear structures. Intraperitoneal injection of 2,7-FAA failed to induce tumors at some sites where tumors had occurred in the animals that ingested the 2,7-FAA; these were the sixth cranial nerve, brain, kidney, and eye.