A simplified micro double-diffusion agar precipitin technique.

Abstract

Abstract Wadsworth's micro double-diffusion agar precipitin technique is presented as a modified, complete procedure suitable for frequent use in unspecialized clinical and bacteriologic laboratories. This procedure has proved more convenient, and far more sensitive and economical in every sense than conventional Ouchterlony methods. Details are given for setting up the test and for washing, staining, and preserving the precipitin reactions.