Analysis of Interacting Cell Populations in Cultures of Marrow From Patients With Neutropenia

Abstract

Abstract Utilizing an adherence separation procedure, marrow cells from six neutropenic patients were studied in cell culture for colony-forming ability (CFU-C), and for colony-stimulating function of the adherent cell (A-C) population. Assays on two patients showed reduced CFU-C and A-C function, while assays on three patients yielded normal CFU-C and A-C function; studies on the remaining patient exhibited reduced CFU-C in the presence of normal A-C function. The demonstration of these various abnormalities in marrow cell culture from neutropenic patients suggests that the in vitro techniques have relevance to the study of granulopoiesis in vivo.