PubMed · 1988 · 45 citations · 0 references
Life SciencesBiologyCell BiologyInsect PhysiologyParasitologyProtozoologyChagas DiseaseHost-parasite InteractionsParasitic Disease PathogenesisParasitic ProtozoaProtozoal InfectionsProtozoan Host-parasite InteractionsPhosphodiesterase InhibitionPhosphodiesterase RegulationPhosphodiesterase SignalingTrypanosoma Cruzi InfectionTrypanosoma Cruzi Life CycleTrypanosomatid-host InteractionsTrypanosome InfectionsTrypanosomiasis Pathogenesis
The addition to epimastigotes cultures of T. cruzi, of either cAMP, monobutyryl-cAMP, dibutyryl-cAMP, 8-Br-cAMP (at 2 mM each), or the cAMP-phosphodiesterase inhibitor, papaverine (0.2 mM), promoted the in vitro differentiation of these parasite forms into metacyclics. This effect of cAMP may also be exerted in vivo in the insect vector, since cAMP was detected in the urine and in the Malpighi secretion fluids of Rodnius prolixus.